What are the impacts if Colombia signs partnerships with Brazil, Vietnam and other producers, and all take coffee from the N.Y?

More João Batista Ferreira's questions See All

Is that possible to do an impedance measurement without FRA module?

One of my colleagues said, "the impedance you can measure with the three electrodes and with the specific sequence varying the frequency" Is it possible to configure the potenciostat without the...

21 February 2021 418 2 View

Can you use MTS assay with cells cultured in media containing phenol red?

I am trying to run a cell viability assay with LN229, but these cells are cultured in DMEM which contain phenol red. Is the MTS assay sensitive to the additional of phenol red in the media? If so,...

03 February 2021 9,478 2 View

How big the Balb/c mice spleen after immunization?

I am working with Balb/c mice, and in my experiment, I have to inject mice with antigens, then get their spleen and collect the splenocytes for fusion. Recently, I get into trouble with the mouse...

04 January 2021 3,591 3 View

How to convert Terapeutic Human Doses to Cell Experimentation Doses?

"Toxicological evaluations revealed that Cur is found to be pharmacologically safe, even up to 12 g per day, as reported by several animal studies and in phase-I clinical trials Similarly,...

07 December 2020 3,012 1 View

How to measure acid values of unsaturated polyesters?

I've been using the standart AATM 109-01 (2006) procedure to determine the acid values of unsaturated polyesters. In this procedure solutions of Phenolphthalein and Potassium Hydroxide in etanol...

11 November 2020 7,768 6 View

CAS solution whit PIPES ?

To prepare CAS solution for siderophores assay, the protocol use anhydrous piperazine. Can I make this solution using PIPES ? And how? Thanks The protocol i'm following is:...

08 November 2020 2,751 2 View

Is it possible to re-use a MK801 20uM solution when used as a bath for fish?

We are exposing zebrafish to an MK801 solution and we are thinking if it is possible to re-use the solution (between 3 and 5 times in a row) in order to decrease the amount of waste produced,...

11 October 2020 7,391 1 View

CRISPR-Cas9: Are there any alternatives to the use of fluorescent PCR and capillary electrophoresis?

We are trying to find alternatives to the use of fluorescent tagged primers and capillary electrophoresis to access somatic activity of the target gene. Thank you!

25 September 2020 4,761 3 View

Does anyone have a reliable protocol to assess cell viability in commercially available GelMA (Gelatin methacryloyl) ?

Hello all, I am trying to asses the viability of stem cells in GelMA (gelatin methacryloyl). Most articles make their own GelMA. However, our team bought a commercially available one. The thing...

13 September 2020 9,086 1 View

Which are the best loading controls for Western Blot excluding the cytoskeleton ones?

I need to use a loading control for Western Blot that neither includes a cytoskeleton protein or a protein that interacts directly with the cytoskeleton. My issue is that I incubate cells with...

27 August 2020 2,735 11 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

Do the introduced salts (NaOH/ HCl) influence the spectroscopic shifts of the fluorescence excitation-emission matrix?

During the resin (XAD-8) fractionation of wastewater salts (NaOH or HaCl) are used for acid or base elutions/pH adjustments. Since the amount of introduced salt is minimal, so is it required to...

08 February 2021 9,489 2 View

Why has my protein transfer using fresh transfer buffer is worse when compared to protein transfer using old buffer?

Hello, I have performed two westerns in parallel, one using fresh buffer (made with old 10X tris-glycine) and the SDS gel was made 4 days prior to western; and the other western was performed...

03 February 2021 9,311 5 View

Why is anionic exchange chromatography more common than cationic exchange chromatography?

Is it true that there are more negative charged proteins?

18 January 2021 8,302 2 View

Please how can I justify my regression results?

I conducted a study to investigate stock market reaction to equity issues announcement and i ended up with 64 announcements and i then used the announcement day returns as my DV and investigated...

14 January 2021 1,127 3 View

Can you help with the implementation of two different model of deep learning for stock market prediction?

I have stock price data(Open, high, low, close), I want to do prediction by combining two different models like CNN with others. Dataset will be same, what are the ways that I can use for...

04 January 2021 1,845 3 View

Why does my Giemsa staining solution stay in the filter?

I wanted to use commercial Giemsa staining solution and diluted it 1:10 in pH 6.8 Weise buffer. It says to filter the solution, however, most of the stain seems to preticipate in the filter. The...

04 January 2021 5,028 2 View

How can I prepare the erythromycin stock?

I need to prepare a stock of 10 mg / ml erythromycin to check the mic value. I am trying to dissolve 0.1g/10 ml of water but i can't dissolve erythromycin in water. How can i prepare 10 mg/ml...

03 January 2021 9,947 3 View

Thomas Markopoulos

It depends on the final goal and the real reason of this action. If they want to create a new market, investors can diversify their portfolio from New York to Latin America. Global markets can adapt easily in such changes. If the goal is to reduce speculation and volatility through derivatives on commodities, it will not work because advanced derivatives could be created and used in New York.

João Batista Ferreira

the purpose is only to determine the price of coffee and not be at the mercy of the market

Emmanuel V Murray

This is certainly a very positive move.

What are the impacts of future stock exchanges?