How do you modify a GROMACS topology file to incorporate a glycan which has been parameterized separately?

I am following Zhang (2015) Understanding the impact of Fc glycosylation on its conformational changes by molecular dynamics simulations and bioinformatics https://bit.ly/2Hi6TYT . I have...

03 April 2018 360 2 View

What is the best sonicator model available for purchase?

I am charged with buying a new sonicator for my department. It will be used for bacterial lysis for protein purification.

02 March 2017 4,409 2 View

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The Wikipedia page on zinc fingers states that such domains exist; salt bridges reportedly take the place of zinc. However, there is no citation! The only example of a natural zinc-stabilized DNA...

06 July 2016 6,210 1 View

What are some general guidelines for nonspecific DNA sequences adjacent to transcription factor (zinc finger) binding sites?

I am going to be designing DNA oligos for zinc finger sites and I need to avoid edge effects. Are there general principles for the composition of nonspecific flanking sites? I've seen some groups...

07 August 2014 1,872 3 View

Heparin LC column memory: use of detergent to get complete elution?

I am trying to develop a heparin affinity column method using a 100mM to 1M NaCl step elution. My protein of interest actually elutes at a 0.5M NaCl step, but though it elutes in a nice, sharp...

08 September 2012 4,057 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

It is necessary to reflux the Schiff base and metal salts to obtain metal complex??

24 February 2021 9,166 3 View