Michael Cukan

6 Questions 12 Answers 0 Followers

Questions related from Michael Cukan

How do you modify a GROMACS topology file to incorporate a glycan which has been parameterized separately?

I am following Zhang (2015) Understanding the impact of Fc glycosylation on its conformational changes by molecular dynamics simulations and bioinformatics https://bit.ly/2Hi6TYT . I have...

04 April 2018 466 2 View

What are the harshest conditions (pH, temperature, salt) a bacteriophage can withstand (i.e. survive and retain ability to infect)?

I seem to have selected phage that have lost the ability to infect bacteria. The best case scenario is these phage are bound so tightly to my target (which is ~50 kDa) that they have lost the...

10 October 2017 1,861 1 View

What is the best sonicator model available for purchase?

I am charged with buying a new sonicator for my department. It will be used for bacterial lysis for protein purification.

03 March 2017 4,526 2 View

Can someone name examples of functional Cys2His2 domains that fold into the characteristic ββα fold in the absence of zinc?

The Wikipedia page on zinc fingers states that such domains exist; salt bridges reportedly take the place of zinc. However, there is no citation! The only example of a natural zinc-stabilized DNA...

07 July 2016 6,295 1 View

What are some general guidelines for nonspecific DNA sequences adjacent to transcription factor (zinc finger) binding sites?

I am going to be designing DNA oligos for zinc finger sites and I need to avoid edge effects. Are there general principles for the composition of nonspecific flanking sites? I've seen some groups...

08 August 2014 1,962 3 View

Heparin LC column memory: use of detergent to get complete elution?

I am trying to develop a heparin affinity column method using a 100mM to 1M NaCl step elution. My protein of interest actually elutes at a 0.5M NaCl step, but though it elutes in a nice, sharp...

09 September 2012 4,138 3 View