What are the effective materials for its capacity on the absorption of phenolic compounds excreted from explant cut, to prevent the occurrence of browning during tissue culture and micropropagation experiments?
Dear Moslem Abarian , if your purpose is to adsorve the phenolics released to culture medium, use activated charcoal (AC). If you purpose is reduce the effects of phenolics, you have two main ways: the chemical and mechanical. The first include the use of ascorbic and citric acids, also AC.... The mechanical way is basically referred to continues subcultures. A third alternative is also possible: the biological, through the use of juvenile explant sources. In my experience, I don't obtained any succes with the use of chemicals; only using young explants and continuous subculture, I have succeeded in some extent. Good luck
@ Ricardo Julian Licea-Moreno , hey, Dear, thank you, In my research, for prevention of occurrence of browning from explants, the Poly vinyl pyrrolidone (PVP), activated charcoal and A consecutive and quick subculturing were applied, so (PVP) was The most effective, suitable and cost-effective...
Good luck :)
Did you want to research on the specific phenols? Maybe that also would be an idea. Might be there is a counter substance to feed into the explant. Just a thought...
@ S. Wenkart .. hey, Dear, I worked most about aromatic and phenolic compounds and biodegradations, but in new my research I was looking an effective method for prevention of occurrence of browning and reduce the effects of phenolics during micropropagations for obtaining higher efficiency...
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