These two words are not specific to plant tissue culture, because hardening of cuttings is a usual practice in plant propagation. It seems that acclimatization is more common in various publications. However, in some papers, hardening is considered as a stage before acclimatization.
Hi, Ali, actually, these two term mean largely the same: plant adaptation during transfer from in vitro to soil. Such transfer include high humidity, low light etc.
Dr, Ali
Acclimatization for Date Palm mean Healthy plantlets of 10-15 cm length, with shoot base of 15 mm or more diameter and adequate rooting should be transferred to pots. Planting operation should be completed as quickly as possible after washing off all traces of culture media and disinfecting with broad spectrum fungicides (1 gl-1 metalaxil or any other fungicide solution 5 minutes before planting). A sterilized medium comprising peat moss,vermiculite and coarse sand in the ratio 1:1:1 is ideal for soil transfer.
to irrigate immediately with 50% Hoagland solution or 10% MS solution to avoid
dehydration of plants. Containers and potting media must be adequately cleaned
and sterilized. Moisture content of the medium must be regulated to minimum, and
relative humidity of the growth tub must be maintained high.
After progressive reduction of humidity to ambient levels, plants are exposed
to the greenhouse conditions for further hardening. Later the plants are transferred
to bigger pots before transferring to nursery.
this mean that Acclimatization be before hardening plant and hardening Hardening is the last step for the transfer of the plant to the soil .
Hardening is the last step for the transfer of the plant to the soil there may be similarities but hardening tougher on the plant and there is another process which precedes these two steps namely pretreatment (in tubes) include adaptable plant against stress
Hi Ali
This two terms are the same and they are used interchangeably
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