Which software to use to statistically analyze Telomere Length_TRF_Southern Blot?

I am trying to measure telomere length in an ant species using the TRF method, which is a Southern blot technique. Currently, I am struggling with analyzing the images and would appreciate any...

29 January 2021 604 3 View

Are researchers less able to research with age?

In your opinion, how old is the researcher feeling that he is unable to absorb and retain information highly in light of the pressures of life and the huge technological development that makes us...

05 August 2019 3,429 68 View

What are the main reasons of the appearance of rarefactive solitary wave ?

I have used a model and i got a rarefactive solitary wave but i do not know if this is an advantage or disadvantage and what are the reasons of the appearance of such waves

12 April 2019 9,935 3 View

What is the difference between using KLH as carrier protein with peptides and KLH as antigen without peptides?

Hello, I am trying to inject KLH in chicken as antigen not conjugated with protein. is it correct to think that KLH is used as antigen? i know that KLH is well known for its potent and safe...

06 April 2019 4,265 4 View

When performing 0.7% agarose gel electrophorsis why there is no difference between a control DNA sample and that exposed to ionizing radiation?

07 November 2018 2,760 4 View

What is the differenc between lymphocyte subset can be detected by flow cytometry and MACS technique?

Lymphocyte subset

25 July 2018 5,762 6 View

What is the cause of failure of detection of significant quantitive trait loci ?

excuse me for that as recently joined this field and trying to make my way. I have a data set of 1545 SNP markers from NGS for many traits of 63 individuals (F2 intercross) the genotypes analyzed...

28 March 2018 7,913 4 View

Why dna not exit from the well of agarose gel

i have extract dna of bacteriophage and make gel electrophoresis and dna apper in the well only why not exit??

20 October 2016 2,754 5 View

How to measure G-mass protein in laboratory?

13 February 2016 784 1 View

Dose anyone have an article about sclerotium sp. metabolites?

Agriculture science

15 August 2013 765 3 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

Separated objects vs Non-Manifold Assembly?

I'm trying to do a simulation between the Maxilla and the Mandible I converted the Dicom file from the CT scan to a CAD module and module the articular discs via 3d software. I have two options...

08 February 2021 8,731 2 View

How to perform CV by using potentiostat?

I have deposited a metal oxide thin film of glass plate, A conductive thin film was also deposited on the glass substrate before the deposition of metal oxide film. How can I perform its CV from a...

01 February 2021 7,710 3 View

Do drugs that are injected i.t in rats reach the supraspinal system and how long does it take to clear them from spinal cord?

I am injecting intrathecally (between L5-L6) , in rats, cannabinoid analgesics and I was wondering if they can reach the brain and have effects there as well ? Also I need to pretreat with...

07 January 2021 5,397 4 View

What is the cause of abnormal breathing in anaesthetized rats?

Is anyone able to tell me why my anaesthetized rats often do a 'double breath', or diaphragmatic breath, like a hiccup (see video). This often begins after 1-2 hours under anesthesia, and can...

06 December 2020 2,514 5 View

Pearsons correlation sample size HELP?

I'm conducting a correlational study about "is there a relationship between depression, acceptance and quality of life among spinal cord injury patient?" But I am having an issue determining the...

06 November 2020 9,798 10 View

Which anesthesia system should I buy to perform stereotaxic surgeries in mice?

I am trying to start a protocol involving bilateral cannula implantation in specific brain regions in mice (mPFC, hippocampus). For that, I need to buy an anesthesia system. I found many...

06 November 2020 3,613 3 View

Plated Secretory Tissue from Udder--What type of cells are these?

Hello all! I plated some secretory tissue from the udder of a dairy cow. I am trying to isolate mammary epithelial cells. I found these cells on one of my plates, are these developing mammary...

28 October 2020 7,768 3 View

Preparation of mouse thymus for Flow Cytometry?

Hi, I'm trying to develop a protocol for prepping thymus tissues in mice. We will be using NSG mice in our lab and the goal is to get good consistent single cell suspensions to stain for FACS....

26 October 2020 10,006 3 View