I have performed the HPLC analysis of methanolic extract of plant Artemisia scoparia and collected total 12 peak (each containing one or more lead compound). Now I want to perform LC-MS analysis of these collected peaks. What conditions like solvent, mobile phase, column temperature, wavelength etc. should be used to perform effective analysis?
Why can't you use the same solvent system (note the exception below!), gradient method, wavelength, and so forth, that you used to collect the peaks? The "MS" part of "LC-MS" is simply another detector. Aside from buffer compatibility with the MS, so long as the chromatography is good, the detector doesn't matter.
The only possible problem I can see if if the mobile phase you used to collect the peaks contains inorganic buffers such as phosphates, sulfates, sodium salts, and non-volatile organic buffers such as citrates.
Check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2766790/
Also check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838194/
Kindly visit..
https://www.researchgate.net/deref/https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC2766790%2F
Whereas, Total LC/MS sample preparation must take into consideration both the mobile phase (containing the analyte) and stationary phase (packing material or support). Soluble and insoluble matrix components should also be considered and whether they might interfere with the analyte's final elution from the column.
Actually, The difference between traditional LC and HPLC is that the solvent in LC travels by the force of gravity. In the application of HPLC, the solvent travels under high pressure obtained by means of a pump to overcome the pressure drop in the packed column, which reduces the time of separation
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