How to Implement Elastoplasticity in a PCL model in COMSOL Multiphysics?

I am using COMSOL Multiphysics 5.5. I am trying to simulate the tensile test of PCL scaffolds. However, regardless of the strain, I have the elastic region all the time which is not true when it...

23 February 2021 4,050 1 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View

How to do qualitative field research in a hostile environment?

It is much said about doing qualitative research and field study. I'm curious about your hints/experiences in doing ethnography in hostile environment touching sensitive topics (marginalized...

30 November 2020 4,151 14 View

What is the scenario for the gold price?

What do you think about the further growth of gold price? Do you think that the upward trend is coming to an end? Or may be we observe a temporary turbulences caused by the US presidential...

23 September 2020 1,344 4 View

How to make a one-dimensional SOM (Kohonen) map?

Could you please help me and give some tips on how to design trajectory/line using a one-dimensional Kohonen "map"? I need it to my article on bankruptcy but as I'm not statistician I can't handle...

23 September 2020 6,834 2 View

What's the acceptable value of _refine_ls_shift/su_max for Rietveld refinement?

Hi, my background is in single-crystal X-ray diffraction. Now, I am carrying out the so-called Rietveld refinements in GSAS-II and am getting quite large _refine_ls_shift/su_max values (ranging...

01 September 2020 7,012 2 View

How can I start a quantum chemistry calculations with Gaussian with modified basis set?

I want to run geometry optimization in Gausian. Currently I am running it this way: #N B3LYP/3-21G OPT NOSYMMETRY 0 6 Dy 0.00000000 0.00000000 0.00000000 F 2.14203091 0.10643632...

27 June 2020 2,912 9 View

A queueing model for the cloud computing centre?

I'm considering an analytical model for the cloud computing composed of: - input load balancer (LB) with M/M/1/K - P (PH) physical machines with hypervisors, each able to host V (VM) virtual...

18 November 2019 2,356 3 View

No title

07 November 2019 2,121 1 View

Suitable PhiX concentration for diversified libraries (MiSeq v2, Nextera XT)?

Dear All, could some please suggest proper PhiX concentration for multiplex Illumina sequencing with use of MiSeq kit v2 and Nextera XT. samples #1-11 are highly similar - around 50-56%GC sample...

07 September 2019 2,793 1 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can any one provide information about medicine, epidemic and critical issues or it is mandatory to be an expert ?

Due to the pandemic of coronavirus, the most source that the people depend on was social media. There is no guaranteed solution that is biased from political opinion. An example of that the...

01 February 2021 7,567 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

Is there a way to cut the restriction enzyme only the desired part?

Hello, I am currently making a luc gene. The primer was ordered and the band amplified with pcr was gel extracted. Restriction enzyme was treated to make the insert sticky with the vector......

18 January 2021 4,357 3 View

Questions on data cleaning and imputation in longitudinal studies?

The study involved mathematics students completing a survey with several scales at three time points. 1) What is a reasonable threshold for missing data to remove an observation? Does this...

04 January 2021 5,582 1 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

01 January 2021 3,271 4 View

Do you think knowledge should be in equal access to everyone ?

everyone knows the importance of knowledge but knowing this too why there is a restriction in getting access to it what is reason behind this.

23 December 2020 2,934 6 View