What are the concentrations (or ratios) of different cytosolic and membranous carbonic anhydrase isoforms in cell?

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Column for purification of rat monoclonal antibody (kappa chain)?

I have 1L of hybridoma supernatant without serum from a rat monoclonal antibody. I would like to purify it using a binding affinity column for rat kappa chain. Could you please tell me where I...

29 November 2020 3,615 3 View

How to separate over expressed protein?

I have to identify the protein responsible for some activity in microbes and have seen over expressed band in native page. how can i purify it for protein sequencing.

21 October 2019 3,822 2 View

Which one is better: Matrigel or Geltrex?

Has anybody tried practically both substances: Matrigel and Geltrex? I found some literature in the Internet about the differences and similarities between those two substances. Maybe someone...

06 July 2018 1,496 3 View

Is this formulation suitable for the i.p. injection?

I made a formulation which includes 5% dextrose, 10% Cremophor EL and 10% Ethanol (aqueous solution, plus tested compound). It was used for oral administration of compound. Is this compositions...

15 December 2017 7,535 7 View

What is the best tissue clearing solution?

I want to make spheroids more clear for confocal imaging. What are the possible ways to clear them? Maybe somebody tried several different commercial solutions or home-made ones and could suggest...

04 October 2017 2,655 6 View

What is the height of cancer cells?

What is the height of cancer cells growing in 2D cultures? I am mostly interested in human pancreatic BxPC-3 and murine breast cancer 4T1 cells.

22 September 2017 2,169 2 View

How to show that ultrasound exists in the cel medium

We are applying ultrasound for live cells grown in multi-well dishes. There is no a noticeable effect on cell viability. How to show that there is an effect of ultrasound at all in the cell medium?

20 July 2017 3,592 2 View

Where is it possible to find a list of certified analytical labs in EU?

Where it would be possible to find a list of accredited testing labs for phamaceuticals in EU (ISO/IEC 17025)?

21 January 2017 4,362 3 View

How can I separate aliphatic and aromatic hydrocarbons by solid phase extraction?

I am looking for a sample preparation technique for total petroleum hydrocarbons that can separate the aliphatic and the aromatic components for GC analysis.

23 October 2014 5,587 10 View

What is the best method for measuring endothelium function?

See above

21 September 2014 541 20 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

Can i do protein expression of Toll-like receptor 4 cloned in pET-22B in E.coli cell?

Can

08 July 2024 6,725 4 View