Has anyone systematically looked at the risks of inhaling particles containing gene-edited human cells?
I am looking for a way to calculate the number of water molecules that are in a hydrogel sample.
16 May 2024 7,064 3 View
I am wondering what folks have tried for alternative methods of detecting hepatitis B core antigen/capsid. Dako used to make an antibody that was very effective in this and widely used in the...
28 April 2024 3,860 1 View
Hello! I'm in the first stages of my thesis and, right now, I'm developing my research aim and objectives, as well as their respective research questions. I would like to study the significance of...
26 November 2022 5,760 3 View
Hi, I would like to measure the halo size of certain antimicrobial inhibition halos and was thinking about using ImageJ. However, I have tons of plates. Do you know of any plugin that can detect...
29 October 2022 4,800 3 View
Does anyone know the best way to analyse this set of questions using SPSS? For reference, my study is comparing the responses of university students and non-university students.
22 May 2022 719 3 View
I am looking anew into the area of what used to be called "scalable autonomy." I worked in this space briefly around 2014. Now, though, I haven't been able to find much associated with the terms...
07 February 2022 9,338 2 View
Hello! My HSQC and H-NMR spectra are not agreeing about the intensity of certain signals. One peak in HSQC is much weaker than in my H-NMR spectra (same sample), and there are no peaks in front...
25 January 2022 1,424 0 View
I prepare in-house media in Duran glass bottles, and am trying to find a way to seal them to prevent any tampering. I currently use shrink plastics post-sterilisation (either autoclaving or filter...
09 June 2021 8,105 1 View
I’m currently trying to develop a 10 SSR marker multiplex for allelic screening using the universal tags and the method (iii) from Blacket et al. (2012 - DOI: 10.1111/j.1755-0998.2011.03104.x )....
14 March 2021 3,069 1 View
Purchased kit to run on old iCycler but directions did not have a method. Only said to use CFX Manager Software and select the appropriate thermo-protocol for the detection or quantification of...
13 November 2020 9,123 1 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View