What are the best primers to amplify 18S and 28S region of monopisthocotyleans and polyopisthocotyleans ?

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Noor Edan Jarad

Article PCR Primers for metazoan nuclear 18S and 28S ribosomal DNA sequences

Jean-Lou Justine

For 28S - the method is well known.

See this 2001 paper

Aashaq Hussain Bhat

For 18S , see the attached papers.

Julio Cesar Cenci de Aguiar

Dear Amit,

I think you should test and find out what would be the best primers to amplify those regions of DNA of the parasite you are interested in. This can vary, according to the parasite, geographic region and so on. I have tested and drawn some for my needs, one of which has already been made available in a recent publication (http://linkinghub.elsevier.com/retrieve/pii/S1383576917300776).

Best regards!