What is the best stain for monogeneans?

More Amit Verma's questions See All

What are the best primers to amplify 18S and 28S region of monopisthocotyleans and polyopisthocotyleans ?

Helminth parasites

11 December 2017 2,861 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View

Kubilay Doğan Kılıç

"A quick and simple method, usable in the field, for collecting parasites in suitable condition for both morphological and molecular studies" from Justine et al. may help you.

Edit:My apologies to JL Justine for writing his name wrong.

Jean-Lou Justine

I agree ! Although I don't know who "Lustin" might be...

Article A quick and simple method, usable in the field, for collecti...

Data 105 Justine Briand Bray - Quick and Simple Method - Parasito...

Kristen Trevey

I used diluted Gomori's trichrome for Gyrodactylid monogeneans. Dilution eliminated the need for unpleasant clearing agents like xylene.

Chahinez Bouguerche

Ii use acetique carmin for Polyop, here's a view of carmin stained specimen