HPLC-MS/MS
Lately I keep getting bad resolution with my results. I changed the parameters of MS and MS(n) and the Max Res Scan in terms of average, scanning range, target mass, etc.
I am using Bruker for that purpose.
Hi Norah -
Can you give us some more details? First of all, do you mean chromatic resolution or mass spec resolution? Are you confident with 'identifying' known proteins? Are you looking at intact proteins or looking at digests? Are you experienced with tuning and calibrating the instrument? Does the mass spectrometer behave well with tuning standards and only with your analytes (i.e., is it a problem with your sample, with you, or the instrument)?
If you're not experienced with the effects of changing MS parameters, this can get you way off the track!
:)
Hi Norah -
Can you give us some more details? First of all, do you mean chromatic resolution or mass spec resolution? Are you confident with 'identifying' known proteins? Are you looking at intact proteins or looking at digests? Are you experienced with tuning and calibrating the instrument? Does the mass spectrometer behave well with tuning standards and only with your analytes (i.e., is it a problem with your sample, with you, or the instrument)?
If you're not experienced with the effects of changing MS parameters, this can get you way off the track!
:)
you have to work on mobile phase used. as there is no details of hplc conditions so, it will be hard say directly. could u please explain in details regarding column conditions. if you want resolution you have to decrease the amount of methanol from to the mobile phase composition and have to increase the weak mobile phase. you can also have option of gradient programing.
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