Neutrophils and eosinophils are both in the granulocyte gate, neutrophils are CD16 positive and eosinophils are CD16 negative. CD15 is expressed both on eosinophils and neutrophils. You can even distinguish eosinophils in unstained samples by their autofluorescence
Hy Massimo. If I am on time to help... Neutrophils: CD68, CD15; Eosinophils: Siglec-8 (the human counterpart of Siglec-F); Dendritic cells: this is tricky... there are several populations of DCs in blood. I worked a lot with this kind of cells and our approach usually was to exclude other lineages (CD3, CD56, CD14, CD19 in the same colour; there are mixtures of these mAbs usually called Lineage exclusion kits) use HLA-DR and select those with high expression. Then you should use CD33 and CD123 and CD16. You can find 3 populations of these cells usually... If you want I can send you some papers with the procedure.
Neutrophils and eosinophils are both in the granulocyte gate, neutrophils are CD16 positive and eosinophils are CD16 negative. CD15 is expressed both on eosinophils and neutrophils. You can even distinguish eosinophils in unstained samples by their autofluorescence