I've been working on MCF7 cell lines for nearly 7 months now. Today while checking one of the flasks, I found its media color dark green and all cells floating dead.
I've trypsinized this flask 3 days ago and transferred the cells to a new T25 flask. I changed the media the following day and the cells were super healthy and fine (40% confluent). Today, I was about to change the media but I found its color dark greenish.
I wonder what might be the cause this to avoid it in the future.
P.S. Water bath and incubator were cleaned 5 days before the incident. Media has penstrip (1x). No obvious micro-organisms were noted under the microscope.
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