Her, I have a Dextran ( mw : 15, 000) , a bioplymer polysaccahride, and i m perfoming the hydrolsysis part by acid treatment & higher temp. to get desired molecular weight of polysaccaride ( mw 1500 to 800 dalton).During the time of acid hydrolsis, huge numeber of glucoses ( monomer & dimer) are also forming along with our desired molecular weight dextran. It is mandtory for me to remove the gucose. Kindly suggest me what are the best method for separtion of gluose from polysaccharide mixture.
Hi Sudhansu
You may try any qualitative chromatographic technique for separation of glucose monomers.
Dear madam, can u sugeest me what kind of chromatographic resin are suitable for separation of glucose or monomeres from my polysaccharide mixture, kindly share few protocols related to this issue.and mentioned the name of specfic resin for glucose separation
There are cellulose membranes on the market I suggest cut off Mw 500Da, it it simple and does not require sophisticated equipment. We used such system when we purified oxidised dextran from low molecular weight oligomers and side products. Article Oxidized Dextran as Crosslinker for Chitosan Cryogel Scaffol...
Article Preparation and physicochemical characteristics of cryogel b...
Dear Dmitriy, Than q so much for sharing this information, even i gone through your attached articles also, but unfortunately those articles full length are not available,time being , is it possible from your side to share the detail procedure , like how i need to perfor this method for purification of monomers and dimeres from the polysaccharidme mixture.
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