Sudhansu Sekhar Choudhury

8 Questions 14 Answers 0 Followers

Questions related from Sudhansu Sekhar Choudhury

What are the best and possible methods fro separation of glucose molecules ( monomere & dimer) from a polysaccharide mixture?

Her, I have a Dextran ( mw : 15, 000) , a bioplymer polysaccahride, and i m perfoming the hydrolsysis part by acid treatment & higher temp. to get desired molecular weight of polysaccaride (...

06 August 2019 2,994 4 View

How to remove the endotoxin from polysaccharides or oligosaccharides?

I have ceratin biologcal samples, which are hydrogenated oligosaccharides and conjugated with iron. it is inject bale product, but I am getting high concentration of endotoxin level, can anybody...

01 February 2019 8,115 2 View

Can anybody suggest me how to do E-rosette inhibitionand lymphocytotoxicity assay in antithymocyte immunoglobulin (ATG) in a descriptive manner?

Hello I purified a specific antibodies (polyclonal) named as antithymocyte immunoglobulin (ATG) from immunized horse plasma by affinity chromatography, now i need to do certain potency test in the...

01 June 2018 7,642 0 View

How to coat the isolated human thymocytes in a 96 well microtitre plate for doing ELISA assay?

I isolated the thymocytes from human thymus and preserved in Liquid nitrogen by using 10% DMSO, further I need to coat this collected cells in a microtter 96 well plate for doing ELISa method...

29 November 2017 1,471 2 View

Can we inject isolated dead human thymocytes from thymus into rabbit or horse for development of human antithymocyt immunoglobuline antibodies ?

Her, i am isolating the human thymocytes by using PBS buffer from human thymus by mechanical method, no doubt I am getting good number of cells,further, I am checking immeditely by using tryphan...

17 May 2017 5,954 10 View

Why isolated thymocytes from human thymus getting lysis in PBS buffer?

I am isolating the human thymocytes from human thymus by mechnical dispersion by using both RPMI and PBS, no doubt , I am observing huge number of cells under microscope , but after 5 to 10...

11 April 2017 1,475 1 View

Why isolated human thymocytes from thymus are getting lysis in PBS buffer after a period of time? can we culture the isolated thymocytes by using RPMI

I isolated the thymocytes from human thymus for development antibodies against thymocytes by mechanical distruption only (no enzyme treatment) by using PBS, but i realized that, less number of...

23 March 2017 1,283 2 View

How much quantities of thymocytes (antigen) and how many days need to inject to horse for developing antibodies (antihuman thymocytes)?

I need to rasise antibodies against human thymocytes (ANTI HUMAN THYMOCYTES) in horse by injecting human thymocytes as a antigen, any body can suggest me , how much quantities of thymocytes...

19 March 2017 8,821 2 View