Sudhansu Sekhar Choudhury

5 Questions 8 Answers 0 Followers

Questions related from Sudhansu Sekhar Choudhury

What are the best and possible methods fro separation of glucose molecules ( monomere & dimer) from a polysaccharide mixture?

Her, I have a Dextran ( mw : 15, 000) , a bioplymer polysaccahride, and i m perfoming the hydrolsysis part by acid treatment & higher temp. to get desired molecular weight of polysaccaride (...

06 August 2019 2,956 4 View

Can anybody suggest me how to do E-rosette inhibitionand lymphocytotoxicity assay in antithymocyte immunoglobulin (ATG) in a descriptive manner?

Hello I purified a specific antibodies (polyclonal) named as antithymocyte immunoglobulin (ATG) from immunized horse plasma by affinity chromatography, now i need to do certain potency test in the...

01 June 2018 7,602 0 View

How to coat the isolated human thymocytes in a 96 well microtitre plate for doing ELISA assay?

I isolated the thymocytes from human thymus and preserved in Liquid nitrogen by using 10% DMSO, further I need to coat this collected cells in a microtter 96 well plate for doing ELISa method...

29 November 2017 1,421 2 View

Why isolated human thymocytes from thymus are getting lysis in PBS buffer after a period of time? can we culture the isolated thymocytes by using RPMI

I isolated the thymocytes from human thymus for development antibodies against thymocytes by mechanical distruption only (no enzyme treatment) by using PBS, but i realized that, less number of...

23 March 2017 1,248 2 View

How much quantities of thymocytes (antigen) and how many days need to inject to horse for developing antibodies (antihuman thymocytes)?

I need to rasise antibodies against human thymocytes (ANTI HUMAN THYMOCYTES) in horse by injecting human thymocytes as a antigen, any body can suggest me , how much quantities of thymocytes...

19 March 2017 8,777 2 View