If i want to do my research data analysis what i should start with?

like a guide especially with putting the score for my survey. *my survey will measure knowledge level for the disease population Moreover, then which tool should I use? My study is a...

18 January 2021 3,819 10 View

Can I use 24 wells black tissue culture uncoated plate for cultuing macrophage (THP-1 treated with PMA) ?or I have to coat them?

Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...

05 January 2021 4,214 3 View

What is the ideal cell number for detection of HIF using ELISA ?

Hi All, I am planning to measure HIF expression with different cellular conditions using ELISA kit, from your experience what is the ideal number of cells used for this type of experiment?

07 December 2020 3,066 3 View

Why I got different sizes with polyacrylamide nanoparticles when I measure the size by DLS? I've prepared them with same procedure?

I've prepared nanoparticles by inverse microemulsion technique and I've functionalised the nanoparticles with N-(3-aminopropyl)-methacrylamide (APMA) (+ve charge) to facilitate cellular delivery,...

02 December 2020 8,329 3 View

My protein ladder is diffused and take a lot of time for running electrophrosis?

hello, I am doing western blot for cancer cell and my protein ladder diffused during running electrophoresis and the sample is disappear. also it takes too much time more than 3 hr for migration....

14 September 2020 5,113 4 View

How can I quantitatively analyze a sample with carbohydrate content?

Can anyone -who may have hands-on experience in analytical chemistry or photochemistry- help me how can I quantitatively analyze a sample with carbohydrate content?

09 July 2020 3,663 2 View

How to model VLC V2V communication system in matlab?

I want to simulate what will be BER vs SNR if i sent a stream of bits through VLC in outdoor system; V2X or V2V. any help??

09 February 2020 6,478 4 View

How to remoe imaginary part of Zin in hfss?

i am working on 6 MHz antenna and i want to remove the imaginary part in the input impedance. i used Lumped RLC bounday condition but no sol. any help?

11 September 2018 2,320 3 View

How to import mimics file to ANSYS workbench?

17 August 2016 4,200 4 View

Is it feasible to do cell viability test by counting cells using trypan blue in a 96-well plate? How can I detach cells from wells exactly?

02 January 2016 5,165 6 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View