Reem Abuhejail

4 Questions 3 Answers 0 Followers

Questions related from Reem Abuhejail

Can I use 24 wells black tissue culture uncoated plate for cultuing macrophage (THP-1 treated with PMA) ?or I have to coat them?

Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...

06 January 2021 4,491 3 View

What is the ideal cell number for detection of HIF using ELISA ?

Hi All, I am planning to measure HIF expression with different cellular conditions using ELISA kit, from your experience what is the ideal number of cells used for this type of experiment?

08 December 2020 3,262 3 View

Why I got different sizes with polyacrylamide nanoparticles when I measure the size by DLS? I've prepared them with same procedure?

I've prepared nanoparticles by inverse microemulsion technique and I've functionalised the nanoparticles with N-(3-aminopropyl)-methacrylamide (APMA) (+ve charge) to facilitate cellular delivery,...

03 December 2020 8,536 3 View

What are the assays used to quantify TCA metabolites?

What are the assays used to quantify TCA metabolites to evaluate intact Krebs cycle in macrophage M2?

28 September 2020 7,307 2 View