What are the advantages and disadvantages of using either PAP or ABC method for immunoperoxidase staining?

10 September 2018 0 9K Report

Avidin-Biotin Complex (ABC) method is the most commonly used immunostaining method for peroxidase based immunohistochemistry, may be because of its sensitivity. Whether Peroxidase anti Peroxidase method (PAP) has any advantages over ABC method ?

Any suggestions on which one would suit for tyrosine hydroxylase (TH) staining in the mouse subtantia nigra and/or striatum?

Please help. Thanks much.

Badges
Science topic

Similar topics
Psychology

More Vidyadhara D J's questions See All

Can anyone recommend a scale to measure Bloom's taxonomy of learning styles?

I am looking for recommendations of scales (in English or Spanish) to measure learning styles according to Bloom's taxonomy of learning. Thank you in advance!

09 February 2021 1,419 8 View

What is the reason for using Glass Impingers?

Impinger bottles are used at some applications, where the gas is cooled and the condensate is dissolved in a solvent contained in the impingers. I have always seen impingers made of glass. Why not...

03 February 2021 6,959 2 View

What could be exact way to remove invasive Prosopis juliflora from arid and semi-arid tropics of world where species is considered disaster ?

Prosopis juliflora is considered a noxious weed but its eradication is very costly inhivitive if no impossible ! We have very strong openion that it's spread could be chacked only if it's pods are...

25 January 2021 4,433 7 View

What is the future of international business?

31 December 2020 5,562 40 View

What is the future of biogas in Asia?

31 December 2020 8,821 16 View

Around 10% of global carbon emissions is contributed by apparel industry?

27 December 2020 4,784 3 View

Does PhD by publication route is available to all or limited to university staff in India or abroad?

27 December 2020 5,315 4 View

Impact of COVID-19 on Children and Families?

26 December 2020 5,894 30 View

What country in Asia produces the most renewable energy?

25 December 2020 8,762 24 View

Which country is the largest producer of solar power in Asia?

25 December 2020 5,836 5 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View