What are suitable housekeeping gene(s) to normalize gene expression profiles in bronchoalveolar cells isolated from normoxic and hypoxic mice?

More Liberty Mthunzi's questions See All

How can I manipulate the axis of graphs in jamovi software, I want to write per m squared as a superscript?

I want to write per m squared as m-2. I know I can hold Ctrl and Shift, then press the + key (0178) for m2 but I do not know what I do to type per as (-)

14 April 2022 6,772 1 View

Using the SVD for fitting a plane given a set of 3D points?

Hi everyone, Im hoping someone can help me better understand the inner workings of the singular value decomposition (SVD). Given a set of 3D points, I can find and fit the best plane by:...

03 December 2020 3,811 3 View

How can one automatically extract drainage basin values from a DEM following Strahler stream ordering approach?

Analysing hydrologically corrected DEM avails an opportunity to calculate morphometric indices such as drainage basin density(DD) (which takes manual approach). Can not DD and others be extracted...

07 October 2020 4,139 3 View

Hello guys. What is the scientific explanation fo C:N ratios of 16.1 in grassland, 14.1 in savanna and 1.4 in dense bushlands encroached by A. karo?

I understand that High litter accumulation and N-fixation in dense bushland are obvious causes of low C:N ratio but why would the grassland have high soil organic carbon than bushlands?

01 January 2018 9,120 3 View

Tamoxifen (4-OHT) use in-vitro?

I have a CreLoxP mouse that expresses cre recombinase, upon treatment with tamoxifen with subsequent deletion of floxed genes. I will isolate alveolar, peritoneal and bone marrow macrophages from...

18 September 2017 7,995 26 View

Immunohistochemical and/or immunofluorescent detection of SMAD phosphorylation: Is it possible?

Hi, I am wondering whether it's possible to detect (by IHC and/or IF) phosphorylation of SMADs (SMAD 1/5/8) in isolated murine cells (alveolar, peritoneal or bone marrow macrophages). Please point...

17 May 2016 3,613 1 View

How can a TV or social media ad generate the same responses among viewers?

I am interested to know the neuroscience, psychological, sociological and political factors behind uniform responses of people to certain ads. Thanks.

24 April 2016 8,123 7 View

BMDMs lift (die?) post removal of L-929 conditioned medium. Why?

I isolate bone marrow from 10-14 wk old B6j wildtype mice. BM is differentiated for 7 days in DMEM supplemented w/ 20% L-929 medium, 10% FBS and 1% HEPES. On day 7 medium is changed for same...

31 March 2016 2,021 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View