Liberty Mthunzi

4 Questions 12 Answers 0 Followers

Questions related from Liberty Mthunzi

Tamoxifen (4-OHT) use in-vitro?

I have a CreLoxP mouse that expresses cre recombinase, upon treatment with tamoxifen with subsequent deletion of floxed genes. I will isolate alveolar, peritoneal and bone marrow macrophages from...

19 September 2017 7,981 26 View

Immunohistochemical and/or immunofluorescent detection of SMAD phosphorylation: Is it possible?

Hi, I am wondering whether it's possible to detect (by IHC and/or IF) phosphorylation of SMADs (SMAD 1/5/8) in isolated murine cells (alveolar, peritoneal or bone marrow macrophages). Please point...

18 May 2016 3,591 1 View

BMDMs lift (die?) post removal of L-929 conditioned medium. Why?

I isolate bone marrow from 10-14 wk old B6j wildtype mice. BM is differentiated for 7 days in DMEM supplemented w/ 20% L-929 medium, 10% FBS and 1% HEPES. On day 7 medium is changed for same...

01 April 2016 2,007 3 View

What are suitable housekeeping gene(s) to normalize gene expression profiles in bronchoalveolar cells isolated from normoxic and hypoxic mice?

I am isolating alveolar macrophages from mice maintained in normoxia and hypoxia (48 hr 10% O2) mice then performing qRT-PCR after processing. May you please suggest suitable housekeeping gene(s)...

25 February 2016 6,556 2 View