What are some good antibody clones to use to stain surface TLR4 and TLR2 on mouse leukocytes?

Yelena Lerman @Yelena-Lerman

26 June 2013 1 1K Report

Specifically, I want to measure TLR4 and TLR2 levels on neutrophils of naive mice.

Alexander V Misharin

TLR2 CD282 6C2

TLR4 CD284/MD2 comples MTS510

Badges
Science method

More Yelena Lerman's questions See All

Who can help me find a collection of works IMPC 2020?

The collection was paid for, but we cannot find it. It contains our publication.

28 September 2023 1,088 0 View

Can you help me identify this Cnidaria ?

Hello, I am studying a zooplancton sample collected in the North of Corsica Island (Western Mediterranea) and I observed this organism that I am not able to identify. I guess it is a Cnidaria but...

10 March 2022 6,125 7 View

Identification in a zooplankton sample ?

Hello, I am studying a zooplancton sample collected in the North of Corsica Island (Western Mediterranea) and I observed this organism. Do you, by any chance, know what is it ? Is it an egg ? A...

06 March 2022 3,837 1 View

Cnidaria identification in a zooplankton sample ?

Hello, I am studying a zooplancton sample collected in the North of Corsica Island (Western Mediterranea) and I observed some organisms that probably belongs to the Cnidaria phylum. I supposed...

06 March 2022 1,990 3 View

Mediterranean zooplankton identification ?

Hello, I am studying a zooplancton sample collected in the North of Corsica Island (Western Mediterranea) and I am struggling with some identifications. If you have any idea of what those...

27 February 2022 6,835 3 View

Could someone help me with a zooplankton identification ?

Hello, may someone help me with this zooplankton identification ? It comes from a zooplankton sample collected in the North of Corsica Islant (Western Mediterranea). Thank you in advance.

24 February 2022 685 4 View

Advanced analysis of categorical and/or dichotomous variables in SPSS. Any recommendations?

Could you suggest any readings, tutorials, youtube videos which would help to consider different possibilities for the advanced analysis (significance, errors, relationships, etc.) between...

03 January 2022 8,231 7 View

What is the difference between Social Science Citation Index (SSCI) and Emerging Science Citation Index (ESCI)?

Are publications in the journals ranked by Emerging Sources Citation Index (Clarivate Analytics) also included in Journal Citation Reports? Do your universities / governments put equivalent value...

31 May 2020 5,974 4 View

Dear collegues, has anybody performed successful staining and fluorescent imaging of the cells grown on matrigel? Do you have technical advises?

The cells are EndoC-BH4. They are grown on matrigel, but they do not seem to be submerged into the gel. I tried to stain them with antibodies, phalloidin, and DAPI after paraformaldehyde fixation...

13 January 2019 4,748 3 View

What is the difference between governance and management?

Is it possible to use them interchangeably?

14 October 2013 9,667 25 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View