Is it possible to synthesis zero valent iron nanoparticles through autoclaving?

Hi, I need to synthesis phytogenic ZVI nanoparticles. So, I want to know that autoclaving iron salt with plant extract is possible or not.

06 July 2020 3,337 15 View

Is there a way to add rows corresponding to the count of mismatched data in an excel column??

Unfortunately I have two lists in one workbook, with inconsistently formatted corresponding data in two lists (on one workbook) When I encounter a mismatch in a column, I need to add rows (for as...

10 March 2020 8,552 2 View

DNA microarray design?

If I wish to design a DNA microarray and I'm going to order the probe oligos from a company. Do I need to design a pair of primers corresponding to each probe for PCR?

18 December 2019 7,864 1 View

Is there a biopolymer with a lower critical solubility temperature than 37 celsius?

Additionally, I would like to use this polymer for 3d printing applications.

23 March 2019 700 3 View

Is RBS sufficient to determine the nitrogen and titanium composition as well as oxygen contamination of a TiN thin film?

I have TiN, NbN, and AlN thin film samples, all of which are grown via MBE on silicon substrates, have a 5-10% oxygen contamination, and are about 50nm thick. Is RBS sufficient to determine the...

11 June 2018 8,972 11 View

How do i calculate the sample size in my research study?

i would like to determine the sample size to use in collection of data among children admitted with a certain condition. how do i go about getting the sample size so as to determine the prevalence...

28 November 2017 9,275 7 View

Which organic solvent is best used in preparing a non-aqueous viscous liquid sample for atomic absorption spectroscopy?

The sample is a liquid lipstick. Also, what is the most appropriate ratio of diluent to sample which would have the least impact to the presence of metals in the sample.

24 November 2017 3,454 3 View

What is your deepest desire?

Recent celebrations of All Saints Day in the christian world and Halloween in the secular world awakened in me serious thoughts of the Last things. At the end of life's journey, when all my...

05 November 2017 2,952 59 View

Stats test help needed please?

I have managed to over think things and utterly confuse myself and some clarity or reassurance would be great :) 1. I want to test the efficacy of one intervention in one group (experimental...

24 October 2017 9,140 9 View

How to separate carotenoids and polyacetylenes in extraction using ethyl lactate or an other solvent?

19 September 2017 8,674 2 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

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22 February 2021 9,932 11 View