Can we modify the QCM for the potentiometric biosensor?

As QCM is used in the electrochemical biosensor but I am unable to find the reports on the use of QCM for the potentiometric biosensor. Is there any standard procedure?

31 December 2013 8,649 1 View

Has anyone working on quartz crystal microbalance (QCM)?

Quartz crystal microbalance is the best method to detect or sense micro molecules such as DNA, RNA, protein and microorganisms like bacteria and fungi.

10 November 2012 2,652 11 View

Do conserved secondary structures of miRNAs interfere with phylogenetic analysis of miRNAs?

Precursor miRNAs show high degree of secondary structure conservation across diverse organisms. Can they interfere with the phylogenetic analysis?

10 November 2012 3,353 1 View

Do conserved secondary structure of miRNAs interfere with phylogenetic analysis of miRNAs??

Precursor miRNAs show high degree of secondary structure conservation across diverse organism. Can they interfere with the phylogenetic analysis?

10 November 2012 3,489 1 View

If I need to do phylogeny analysis of the miRNAs, is there any special software or a regular phylogeny analysis softwares?

How is phylogeny analysis of these miRNAs done?

10 November 2012 4,771 6 View

What is the exact difference between miRNA and siRNA?

Understanding the post transitional mechanism is a challenging task.

10 November 2012 3,437 9 View

Does anyone have experience with miRNA target prediction tools?

09 October 2012 5,626 4 View

What are recent aspects of ncRNA/miRNA in diagnosing of diseases or predicting the silencing mechanism ?

miRNA is a good tool in post translational regulation....but what are the hot topic of these RNA's?

09 October 2012 4,274 0 View

Did anyone used miRanda software

its a software of miRNA target prediction, I want to use it but before that I want to know about it briefly, whats are advantages & disadvantages?

09 October 2012 9,962 2 View

How are miRNAs classified? How nomenclature of these miRNAs are accomplished?

Understanding the nomenclature & classification of miRNAs is basic task when working with these tools against regulation of different diseases.

09 October 2012 9,362 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Statistics: include groups of respondents made by one person for analysis?

Good morning, I could not find literature about this so I am asking. I am organizing data for a questionnaire (N=100) that foresees some personal questions like level of education. For the aim...

25 February 2021 2,090 4 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View