Can we modify the QCM for the potentiometric biosensor?

As QCM is used in the electrochemical biosensor but I am unable to find the reports on the use of QCM for the potentiometric biosensor. Is there any standard procedure?

31 December 2013 8,649 1 View

Has anyone working on quartz crystal microbalance (QCM)?

Quartz crystal microbalance is the best method to detect or sense micro molecules such as DNA, RNA, protein and microorganisms like bacteria and fungi.

10 November 2012 2,652 11 View

Do conserved secondary structures of miRNAs interfere with phylogenetic analysis of miRNAs?

Precursor miRNAs show high degree of secondary structure conservation across diverse organisms. Can they interfere with the phylogenetic analysis?

10 November 2012 3,353 1 View

Do conserved secondary structure of miRNAs interfere with phylogenetic analysis of miRNAs??

Precursor miRNAs show high degree of secondary structure conservation across diverse organism. Can they interfere with the phylogenetic analysis?

10 November 2012 3,489 1 View

If I need to do phylogeny analysis of the miRNAs, is there any special software or a regular phylogeny analysis softwares?

How is phylogeny analysis of these miRNAs done?

10 November 2012 4,771 6 View

What is the exact difference between miRNA and siRNA?

Understanding the post transitional mechanism is a challenging task.

10 November 2012 3,437 9 View

What are recent aspects of ncRNA/miRNA in diagnosing diseases or predicting the silencing mechanism?

These are post translational regulatory mechanisms. But what are the recent activities going on in ncRNAs or miRNAs?

09 October 2012 9,359 0 View

Does anyone have experience with miRNA target prediction tools?

09 October 2012 5,626 4 View

What are recent aspects of ncRNA/miRNA in diagnosing of diseases or predicting the silencing mechanism ?

miRNA is a good tool in post translational regulation....but what are the hot topic of these RNA's?

09 October 2012 4,274 0 View

How are miRNAs classified? How nomenclature of these miRNAs are accomplished?

Understanding the nomenclature & classification of miRNAs is basic task when working with these tools against regulation of different diseases.

09 October 2012 9,362 3 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Is it possible to remove the cells from a tissue and keep only the extracellular matrix for proteomics?

Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....

14 February 2021 1,602 4 View

Apart from MOFA what other software is available for analysis of a multi-omic study?

Study bringing together genomics, proteomics etc together

02 February 2021 2,856 2 View

How can I reduce run-to-run variation in TMT MS experiments?

I planned an experimental setup using 10-plex TMT with 9 conditions and 1 bridge channel to compare 5 replicates. But since I experienced some run-to-run variation, I was wondering if a better...

31 January 2021 3,405 3 View

Where can I purchase NanoLC emitter fused silica picoTip?

Hi, I'm working with NanoLC/MS for both Metabolomics and Proteomics. We usually make our nano columns using emitters from NewObjective. Unfortunately New Objective, due to the pandemic, is now...

10 January 2021 1,176 4 View

An optimal protocol of EVs isolation for proteomics?

Hi everyone, I am starting a project in analyzing EVs proteomics in cancers. However, the most published articles I have found stated only brief isolation protocols that could not fully be...

17 December 2020 7,600 2 View

Infected plant protein lysate washing out purified GST-tagged protein on agarose resin?

Hi, I am trying to screen infected plant proteomes for targets of a secreted pathogen protein. I have purified the pathogen protein using a GST-tag on an agarose resin. I am using the Pierce...

23 November 2020 7,009 3 View

Dataset: Orbitrap velos CID MS/MS spectra ?

Hi, I want the MS2-CID spectra from orbitrap velos mass spectrometry of organism: human (Mod: C+57) for testing my peptide identification algorithm developed. Large set of orbitrap velos dataset...

16 November 2020 8,261 1 View