What are optimized conditions to determine carbohydrate by using LC/MS ?

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Fatma Akbas

For each system are the conditions different. You have to try out. There are tools to find with less analysis like Placket Burmann design.

Ming Liu

you can try HPLC-ELSD or HPLC-RI

by the way ,the carbohydrate that you want to analysis,how much is the DP?what is the molecular weight?

kuppusamy Chenniappan

Hello friends,

I am having a cc fraction of ethanolic extract of a plant. I want to analyse what type of compounds presents in it. but I did not know about it, the technical assistant said bring some details about the molecular weight of the compound, melting temperature etc...could you help me how to set a analytical profile in LC-MS..Please help me by replying .