How to purify cycle sequencing PCR reaction?

i am purifying cycle sequencing PCR reaction. i have tried multiple methods given in BDT manual (provided with the instrument) .everytime i used 96 well reaction plate for this purpose. 7 wells...

14 February 2021 8,534 2 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

What are the reasons of asymmetrical bilateral knee Osteoarthritis (OA)?

Primary osteoarthritis - is degenerative joint disease without any apparent underlying cause then what are the reasons of asymmetrical bilateral Knee OA?

09 January 2021 7,524 8 View

Zero normal flux boundary condition for shallow water equations?

I am looking for any book/article reference about the mathematical description of zero normal flux boundary condition for shallow water equations. My concern is that for a near-shore case how it...

16 December 2020 9,383 8 View

How to fix the fungus for SEM imaging?

I want to perform the SEM of candida sp. and i want to know that the fixation for the bacteria and fungus are different or same?

10 December 2020 6,169 3 View

How to adapt a mature Questionnaire?

I am looking forward to seek some help regarding adaptation of mature questionnaires. I am new to quantitative studies and wants to use some demographic in my questionnaire, is it necessary to add...

08 November 2020 7,146 5 View

Primer degradation.....what could be the possible reasons for not having PCR results.????

HI! whenever I start doing PCR with a new primer pair that is lyophilized.1st I make 100pmol solution then 10pmole dilution of it as a working solution. I got good results with this freshly made...

01 September 2020 2,114 15 View

Which are the best open source plotting software?

Dear friends, Please suggest few good graph plotting software that are freely available. If it has an contour plotting option, it will be an added advantage. Origin is a paid software which is...

06 July 2020 9,106 15 View

How can I import a user subroutine into Abaqus component in Simulia Isight?

For optimization my material parameter I use Isight software. I have an input file that simulates the material model via UMAT subroutine. How can I import this inp. file in the Abaqus component of...

14 May 2020 3,552 3 View

Name the hydrological models, which can simulate ice dynamics?

Please explain their principles briefly with references and flowchart. What are their advantages and limitations?

12 April 2020 2,718 1 View

How to correctly dissolve metal complex to run molar conductivity?

03 August 2017 9,354 4 View

Suggest any instrument, which can be used to identify the nature of the compounds (flavonoid, terpenoid)?

26 July 2017 6,452 12 View

Can acetic acid be used for organic nanoparticle synthesis?

21 July 2017 6,813 3 View

How can I quantify caustic soda in cleaning products?

20 July 2017 8,047 3 View

Can anyone suggest me how to prepare the sample of Cu-BTC MOF for SEM analysis?

The Cu-BTC mof was synthesized from mixture of water and ethanol

20 July 2017 558 2 View

How can i make a even, thin Polycaprolactone film?

Hello. I'm trying to make Polycaprolactone(PCL) film by solvent casting method. I am dissolving PCL into Chloroform or Dichloromethane. but, only by this, film is too crystallized ( I can see...

17 July 2017 2,639 4 View

TLC retention factors differences from refer journal

10 July 2017 8,149 6 View

I try to improve fluorescence of drug with lanthanide and silver nano particles but i found that in case f absence f SNP is more than its presence?

in most research it supposed that SNPs(silver nano particles ) improve fluorescence thats not happen with me????

08 July 2017 7,981 1 View

Help me with DPPH assay

I think that we should use methanol or DMSO to dissolve DPPH and samples. But my samples (ethanol 25%, 50%, 75%, 96% and water extract) can't dissolve in methanol nor DMSO. What should i do now?

06 July 2017 2,993 2 View

AOT from L1B/L2 MODIS data

How can I extract the AOT from MODIS, without using the ready AOT product? Do I need the Rayleigh path reflectance and how can I calculate it ?

06 July 2017 6,852 0 View