Previously, phytochemicals test were done to analyse the chemical nature /types but now the quantity is less to use. So need any feasibility details available on instrument.
Dear Deepika,
Have you considered the use of HPLC. Please find an application note attached.
Kind regards,
Ade Kujore
Marketing
Cecil Instruments Limited
Milton Technical Centre, Cambridge CB24 6AZ
United Kingdom
email:- [email protected]
telephone:- +44 (0) 1223 420821
fax.:- +44 (0) 1223 420475
web site:- www.cecilinstruments.com
Try with NMR. After the analysis you may recover all of your sample without any loss.
Depends on your compounds. Gcms works great for mono-, sesqui-, and di-terpenoids. Dr Robert Adams compiled a database to allow ID of 2200 cmpds. Wiley sells a database with even more cmpds than that. Mere nanograms can be enough to get a signal and a positive ID in some cases.
LC-MS and NMR are the best instruments for the identification of flavonoids and GC-MS is the best for the identification of different terpenoids.
Depends on the purity of your sample(s). If you have a relatively pure sample (>90% pure), H-NMR and 13C-NMR along with 2D experiments will give you information on the type of compound, structure and stereochemistry. If you have a mixture such as crude extracts, partially purified extracts or column fractions, the choice is HPLC-MS or better HPLC-DAD-MS. For essential oils and other mixtures of volatile compounds GC-MS is the election.
When quantities are small, I would prefer HPLC(or UPLC) hyphenated with Photo Diode Array Detector (PDA) - Mass spectral detector (ESI-MS/MS for example) that will provide UV spectra, possible molecular mass and fragmentation patterns (by MS/MS. Such information will be useful for possible identification.
- Badges
- Science topic