How to purify cycle sequencing PCR reaction?

i am purifying cycle sequencing PCR reaction. i have tried multiple methods given in BDT manual (provided with the instrument) .everytime i used 96 well reaction plate for this purpose. 7 wells...

14 February 2021 8,534 2 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

What are the reasons of asymmetrical bilateral knee Osteoarthritis (OA)?

Primary osteoarthritis - is degenerative joint disease without any apparent underlying cause then what are the reasons of asymmetrical bilateral Knee OA?

09 January 2021 7,524 8 View

Zero normal flux boundary condition for shallow water equations?

I am looking for any book/article reference about the mathematical description of zero normal flux boundary condition for shallow water equations. My concern is that for a near-shore case how it...

16 December 2020 9,383 8 View

How to fix the fungus for SEM imaging?

I want to perform the SEM of candida sp. and i want to know that the fixation for the bacteria and fungus are different or same?

10 December 2020 6,169 3 View

How to adapt a mature Questionnaire?

I am looking forward to seek some help regarding adaptation of mature questionnaires. I am new to quantitative studies and wants to use some demographic in my questionnaire, is it necessary to add...

08 November 2020 7,146 5 View

Primer degradation.....what could be the possible reasons for not having PCR results.????

HI! whenever I start doing PCR with a new primer pair that is lyophilized.1st I make 100pmol solution then 10pmole dilution of it as a working solution. I got good results with this freshly made...

01 September 2020 2,114 15 View

Which are the best open source plotting software?

Dear friends, Please suggest few good graph plotting software that are freely available. If it has an contour plotting option, it will be an added advantage. Origin is a paid software which is...

06 July 2020 9,106 15 View

How can I import a user subroutine into Abaqus component in Simulia Isight?

For optimization my material parameter I use Isight software. I have an input file that simulates the material model via UMAT subroutine. How can I import this inp. file in the Abaqus component of...

14 May 2020 3,552 3 View

Name the hydrological models, which can simulate ice dynamics?

Please explain their principles briefly with references and flowchart. What are their advantages and limitations?

12 April 2020 2,718 1 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View