14 July 2021 0 5K Report

I knockdown SUN1 protein from my Human MSCs and with QPCR I saw an expression of CD73 where as Endoglin and THY-1 were not expressed. where as my control MSC cells still expressed all three genes.

what could they have differentiated into?

More Muhammad Uzair Rehman's questions See All
How combine yolo with Faster R-CNN?

I want a model that is balanced with accuracy or speed, faster rcnn has high accuracy while yolo have fast speed. i am thinking to combine them to get a hybrid model to achieve both speed and accuracy

02 August 2024 3,104 0 View

What should be the sample container for the hydrothermal reaction in a microwave reactor at 180 °C for 10 min at the heating rate of 5 °C per minute?

Suggest one of them 1. Teflon-lined stainless steel autoclave: 2. Alumina (Al2O3) ceramic container

30 July 2024 7,326 1 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,027 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View

Swerling Characteristic functions?

Hello!!! I want to implement the Swerling characteristics functions (CF) directly in MATLAB without using its Fourier integral pairs...the Swerling CFs are actually Laplace Transform of the signal...

23 July 2024 4,925 1 View

Radar Detection Probabilities?

Currently I need to calculate detection probabilities (PD) from RCS data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0 Equation. The idea is based on...

22 July 2024 2,851 0 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View

Radar Detection Probabilities using beta distributed Scattering Cross section?

Currently I need to calculate detection probabilities (PD) from radar cross section (RCS) data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0...

22 July 2024 868 0 View

I want to buy Hydrothermal Synthesis Autoclave from any European company. Can anyone suggest any company inside Europe?

Thanks

22 July 2024 1,143 3 View

Optimal condition for depositing FTO target 95:5% using sputter technique?

Hello I want to know about the sputtering condition of depositing FTO from target 95:5%. I tried with RF sputter in pressure 2.5Pa and 100 sccm Ar at room temperature but it showing no...

21 July 2024 1,680 2 View

Similar questions and discussions
How to extract RNA from neurons?

Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...

23 July 2024 6,783 1 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

18 July 2024 4,183 2 View

In sds page and western blot, non specific expressed protein with very thin band is also there along with thick band of specific protein. why ?

Asper discussion, First time my clonning was failed becuase the first 45 nucleotide was not cloned in pet30 a vector then I choosed the different clone of 1:1 ration fraction, I got the size of...

13 June 2024 7,564 7 View

How to avoid errors while doing qpcr?

I have been doing qpcr for an year now but everytime I am getting errors. Either positive control cq values will vary or there will be no amolification. The knockdown genes are showing increased...

07 June 2024 7,292 1 View

Lentiviral Transduction Issues?

Hi there, I am attempting to transduce cells with the TLCV2 plasmid containing our sgRNA of interest. My transfection of the HEK 293T cells seems to yield positive results as the EGFP is clearly...

06 June 2024 2,131 1 View

How can I select the narrow band filter in Zetasizer nano ZS?

I am working with chitosan/dextran sulphate nanoparticles loaded with rifampicin, which is fluorescent. Dsl analysis are bad and I saw that is necessary use a narrow band filter. How can I select...

05 June 2024 1,324 3 View

My transduced cell can resist Puromycin and 99% express GFP, BUT can not show me the difference in the knockdown. what could be the possible reason?

My transduced cell can resist Puromycin and 99% express GFP, but it cannot show me the difference in the knockdown. What could be the possible reason?

31 May 2024 454 2 View

Does anyone know where this module is in Simulink?

I try to build a three-phase wireless power transmission model and saw this module in an article.

27 May 2024 3,471 2 View

I will like to knockdown two genes using shRNA what is the best method and plasmid I can use?

I will like to knockdown two genes using shRNA what is the best method and plasmid I can use?

21 May 2024 5,796 0 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close