I have to work on various diseases causing genes of Plant Pathogen. In what situation, I can use southern blot and northern blot
Dear Researchers, I have read an article and there are two set of primer pairs. Sense primers and antisense primers. The paragraph from the article is as [ The partial DNA-dependent RNA...
31 December 2019 7,702 7 View
In 3ml, how can I make all these buffers, I have read this paper for SOD activity, Please find below [The 3 ml reaction mixture contained 50 mM potassium phosphate buffer (pH 7.0), 0.5 mM...
06 July 2019 3,836 6 View
Dear All, I need to check the growth promoting effects of some bacterial antagonists. I want to obtain the jasmonic acid and other growth promoting hormones. Then I want to do LCMS. Any step by...
05 June 2019 2,710 4 View
I want to check some metabolites from the root exudates or leaf extracts. Than I want to check the metabolites concentration like Jasmonic acid, via GC-MS, LC-MS or HPLC. Can anyone explain me...
03 April 2019 3,524 5 View
Dear All, Can anyone explain the pairwise permanova for the group 1 and Group 2. The data is given as below: Group 1 Group 2 Sample size Permutations pseudo-F p-value q-value A C 12 999...
11 December 2018 5,516 0 View
I have a haemocytometer with 25 major grids and each grid is divided into 16 small grids, The dept is 0.1mm. I put 10 ml ddH20 in petriplates and rub to get spores. I take 10ul from that 10 ml...
01 February 2018 2,835 1 View
Dear All, I have read several papers like these: twelve strains of fungus Trichoderma harzianum were evaluated against Phytopathogen. So in these case what would be the definition of strains?
10 November 2017 6,435 10 View
Dear All, I want to extract antibiotics and peptides from a fungi. Is there any proper procedure to extract antibiotics from the fungi.
10 November 2017 6,815 3 View
Dear All, I have cultured two fungi one 9cm petri plates. The growth of biocontrol agent is very slow but it has potential to parasitize a plant pathogen. I want to measure the percent inhibition...
10 November 2017 5,113 3 View
I run the Gell for DNA of some of samples. However some DNA bands were thinner some were more thicker in size and some of them were like smear from top to bottom. What could be the reason?
10 November 2017 9,962 4 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
06 August 2024 8,981 8 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).
04 August 2024 8,798 7 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
31 July 2024 5,087 4 View
What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?
31 July 2024 7,921 5 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?
31 July 2024 8,939 5 View