Concentration of chemicals are usually important in any experimemt especially those of life sciences. If you are extracting, the concentrations or dilutions should be reported. Well if u can be more specific maybe with an example I think more answers to your question will come.
For Instance, if concentration of a chemical is 2000 PPM in a stock solution. Then if we change it to 1000 PPM. The concentration of chemical in experiment and solubility will same or different on same scale?
your stock standard must not degrade at your prepared concentration. diluted standard solution at lower concentration starts degradation with respect to time and temperature.you have to study your chemical degradation with using appropriate method.it is advisable to prepare stock standard solution at higher concentration(2000ppm or 1000 ppm), At 1000 ppm concentration your chemical will remain same,
The reaction rate (for example degradation) could be affected by the initial concentration of the solution. This can be clearly observed by photo chemical reactions. At high concentrations we may have lower degradation rate due to the filter effect of the concentrated chemical itself in the solution.
Within solubility and solution stability limits, the concentration of stock solutions is immaterial. What you need to consider is how the solution may be manipulated to test the hpyothesis in a realistic way. You may wish to calculate chemical species concentrations/activities.