I am using SpectraMax 190 Microplate Reader kinetics (Endpoint) for an Assay that automatically corrects Optical density measurements to a 1-cm pathlength with PathCheck Technology.

While using Beer Lambert's law

Absorbance = epsilon * concentration * length

So here A is absorbance of assay (I am conducting multiple absorbance for assay to establish Vo, so which absorbance should I pick for calculation of Extinction Coefficient?)

Concentration (How to find the concentration?)

Length (1 cm)

Thank you

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