I am using SpectraMax 190 Microplate Reader kinetics (Endpoint) for an Assay that automatically corrects Optical density measurements to a 1-cm pathlength with PathCheck Technology.
While using Beer Lambert's law
Absorbance = epsilon * concentration * length
So here A is absorbance of assay (I am conducting multiple absorbance for assay to establish Vo, so which absorbance should I pick for calculation of Extinction Coefficient?)
Concentration (How to find the concentration?)
Length (1 cm)
Thank you