What is the difference between aptamer treated nanoparticles and functionalized nanoparticles?

03 April 2017 7,724 3 View

How to synthesize powdered gold and silver nanoparticles so that they do not agglomerate?

Colloidal solution of gold and silver nanoparticles tend to agglomerate when centrifuged.

02 March 2017 9,198 4 View

How many research papers have been published on "Smartphone based sensing devices" in the last 10 years?

I want to make a statistical collection of number of research papers published on "smartphone based sensing devices" on various fields in the last 8-10 years. How can I get the information on the...

03 April 2016 411 0 View

Can we use plano-convex lens for producing uniform illumination?

A plano-convex lens is supposed to make the light rays parallel along the optical axis of the lens. Does it ensure that the collimated beam falling on a surface gets uniformly illuminated?

03 April 2016 7,006 4 View

How does size of AuNPs affects the adsorption of BSA on the AuNPs?

31 December 2015 6,359 2 View

How can we improve the selectivity of LSPR based sensing in complex biological media?

In complex biological media, many biomolecules can have the affinity to bind with the nanoparticles' surface but when it comes to selectivity, it becomes difficult to control the selective binding...

31 December 2015 1,564 1 View

Where can I find the number of published research papers in a given area?

Can anybody help me in finding the year wise publication record on a given area of research?

31 December 2015 4,971 1 View

Apart from TEM imaging, what are other characterization technique available to ensure binding of BSA protein and Trypsin enzyme on AuNPs colloids?

What are the characterization technique available to ensure binding/ adsorption of BSA protein and Trypsin enzyme on citrate capped gold nanoparticles in colloidal form. It means I want to do the...

11 December 2015 4,093 14 View

How does sensitivity (nm/RIU) differ for LSPR in solution phase as compared to LSPR using nanoparticle substrate fabricated using lithography?

11 December 2015 971 3 View

Can we say Catalase enzyme and BSA protein as biomolecules?

Can we say that BSA protein and catalase enzyme are biomolecules?

11 December 2015 762 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View