What is the difference between aptamer treated nanoparticles and functionalized nanoparticles?

03 April 2017 7,724 3 View

How to synthesize powdered gold and silver nanoparticles so that they do not agglomerate?

Colloidal solution of gold and silver nanoparticles tend to agglomerate when centrifuged.

02 March 2017 9,198 4 View

How many research papers have been published on "Smartphone based sensing devices" in the last 10 years?

I want to make a statistical collection of number of research papers published on "smartphone based sensing devices" on various fields in the last 8-10 years. How can I get the information on the...

03 April 2016 411 0 View

Can we use plano-convex lens for producing uniform illumination?

A plano-convex lens is supposed to make the light rays parallel along the optical axis of the lens. Does it ensure that the collimated beam falling on a surface gets uniformly illuminated?

03 April 2016 7,006 4 View

How does size of AuNPs affects the adsorption of BSA on the AuNPs?

31 December 2015 6,359 2 View

How can we improve the selectivity of LSPR based sensing in complex biological media?

In complex biological media, many biomolecules can have the affinity to bind with the nanoparticles' surface but when it comes to selectivity, it becomes difficult to control the selective binding...

31 December 2015 1,564 1 View

Where can I find the number of published research papers in a given area?

Can anybody help me in finding the year wise publication record on a given area of research?

31 December 2015 4,971 1 View

Apart from TEM imaging, what are other characterization technique available to ensure binding of BSA protein and Trypsin enzyme on AuNPs colloids?

What are the characterization technique available to ensure binding/ adsorption of BSA protein and Trypsin enzyme on citrate capped gold nanoparticles in colloidal form. It means I want to do the...

11 December 2015 4,093 14 View

How does sensitivity (nm/RIU) differ for LSPR in solution phase as compared to LSPR using nanoparticle substrate fabricated using lithography?

11 December 2015 971 3 View

What is the different between R,G,B, linear R,G,B and non-linear R,G,B values of a colour?

10 November 2015 5,426 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

How to do the calculation for super oxide dimutase assay and how to calculate the R squared value in regression equation for catalase assay?

i have the enzymatic antioxidant assay by following the method of Misra and Fridovich, 1972 for super oxide dimutase and Luck, 1974 for catalase assay can any help me how to the calculation for...

11 February 2021 2,241 3 View

CAT assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!

11 February 2021 7,273 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View