Can I get exosomes from cryopreserved whole blood supplemented with concentration of DMSO (10%)?

I read many papers about exosome isolation from fresh blood or frozen plasma, but not for frozen whole blood. I am wondering whether can I get exosomes from cryopreserved whole blood? One of my...

26 October 2020 5,166 3 View

Reliable PGP9.5 antibody to visualize intraepidermal nerve fibres in rat paw skin?

Hi, I'm looking for a reliable PGP9.5 antibody that can visualize INEF in rat paw skin. Most publications with good image on rat paw uses Ultraclone or AbD Serotec's rabbit anti-human antibody...

19 February 2020 979 2 View

Which channel/filter to use on BioRad CFX96 for Thermal Shift Assay?

Thermal shift assay is very new to me so please bear with me. This is a very specific question and it does seem like a lot of people use BioRad CFX96 to do their Thermal Shift Assay using SYPRO...

15 December 2016 3,990 14 View

Is there any commercial anti-EGFRvIII antibody for flow cytometry recommendation?

I want to test the EGFRvIII expression in my cell line. I am wondering if there is any commercial anti-EGFRvIII antibody for flow cytometry? Has anyone have this experience?

20 January 2016 1,183 1 View

Can someone help me with in vitro functional analysis of exhausted CD8+ T cells?

22 February 2015 8,336 4 View

Is the order of impedance modulus value different with the corrosion resistance?

In the EIS tests, Zsimpwin software is taken to fit the results. My results show that the order of impadance modulus value is different from the oder of corrosion resistance (charge transfer...

02 July 2014 8,440 3 View

Can anyone explain the debris/junk in my MEF cell culture?

I seeded MEFs (passage 5), mitomycin C, or UV treated MEFs ( passage 6) in a 96-well plate at a density of 40, 000 cells/well, since I wanted the wells to be very confluent. I always see a lot of...

20 May 2014 435 4 View

Decreases in pH after the addition of DPG to DIW?

pH of pure DPG is around 4-5, DIW around 5.50, but when both are mixed together in 9 part DIW with 1 part vol DPG the pH drops to 3.49. What could be the explanation for this observed change in pH?

26 March 2014 618 4 View

Can I use SPSS to produce symmetric and asymmetric trimmed mean of Ft statistic?

Robust method of Ft statistic

18 December 2012 6,145 1 View

I am teaching design theory in a University in China.I am very interesting in your project, can you send me the full-text?

I am writing a article to introduce the western study on craft.Your article has inested me.

01 January 1970 4,126 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View