Why does the fatigue cracks initiate from surface to interior as the stress level decreases?

It is well known that low-cycle and high-cycle fatigue cracks initiate from the surface, while ultra-high cycle fatigue cracks initiate from the inside. I am very curious why this transformation...

17 January 2021 9,633 6 View

Where can I find these two papers?

14 January 2021 1,572 3 View

After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

How to understand the chemo-mechanical coupling?

Recently, I came into contact with a new discipline named chemo-mechanical coupling. I found that chemo-mechanical coupling phenomena exist in many research areas, ranging from development of...

17 December 2020 1,065 3 View

I am working on membrane distillation model in COMSOL and I have some problems coming in it?

I need to add a latent heat flux of evaporation to the hot side boundary, which is a function of the pressure difference between the cold side and the hot side boundary.However, the pressure at...

16 December 2020 9,890 1 View

Is there any ingredient I an add into formula to slow down gas generation from Vitamin C?

I know Vitamin C is unstable and tends to be oxidized quickly. The problem here is I made a Vitamin C beverage at pH 4-5, and it starts to generate gas in the packaging, especially at high...

13 December 2020 6,055 1 View

Why use STOP solution in ELISA?

At the last step for data analysis, we always use a STOP solution to stop the TMB oxidized by HRP. Why do we have to stop the reaction? Couldn't we measure the color wavelength of...

17 November 2020 5,177 3 View

Why case-controlled study is more bias prone than cohort study?

I noticed from several articles saying that case-controlled study is more bias prone than cohort study (in the context of cancer early detection using molecular marker). I spent sometime to try to...

17 November 2020 1,878 4 View

Can I get exosomes from cryopreserved whole blood supplemented with concentration of DMSO (10%)?

I read many papers about exosome isolation from fresh blood or frozen plasma, but not for frozen whole blood. I am wondering whether can I get exosomes from cryopreserved whole blood? One of my...

26 October 2020 5,166 3 View

How to perform statistic analysis for age range between two cohorts?

Hi, I was reading the paper (MicroRNA Expression, Survival, and Response to Interferon in... ). In Table 1, I'm wondering how did the authors calculate a p-value of 0.29 for Age? The age in Table...

20 October 2020 2,276 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View