What are best method for plasmid transformation into Cupriavidus sp. bacteria? i

More Tharshini S Mahendran's questions See All

What is considered a biological replicate when you are doing an ELISA plate measurement with different cell types?

If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...

17 June 2024 6,705 1 View

Which phosphor antibodies to choose to study the hypo and hyperphosphorylation state of the SRSF1 protein?

I am trying to understand the different phosphorylation states of SRSF1 protein in the cytoplasm vs nucleus and looking for specific antibodies for the phosphor state. As SR proteins include...

11 February 2024 7,242 3 View

Why does sauce(oyster sauce) because water?

the company that I'm working in produces sauce and we notice there is watery issue in our oyster sauce after bottling although the sauce is within its validated shelf life.

09 May 2023 5,977 1 View

Is it necessary to change media after transfecting cells with Lipofectamine3000?

I transfected HCt116 cells with GFP using Lipo3000. I didn't change media after transfection as the manufacturer guidelines say not necessary. However, I noticed a change in the cell morphology...

28 April 2023 6,071 6 View

Tafel analysis clarification and its reference?

I have done potentiodynamic polarization (Tafel) studies for a few surface modified samples. The intercepts and slopes were done exactly by the origin software. Its results show a gradual...

06 April 2023 1,226 1 View

Is Native RNA immunoprecipitation performed without cross linking?

I am trying to study the RNA-protein interactome of a specific protein using IP studies. I need to use native IP for my purpose and wanted to know if native RIP is without cross-linking study and...

01 February 2023 915 0 View

Is loading equal volume of protein in SDS PAGE correct?

I am comparing the protein expression levels in subcellular fractionations (Cytoplasm and nuclear). If I load an equal volume of both extracts (15 ul each to individual wells), is this still valid...

26 January 2023 6,257 3 View

How to use CRISPOR software for design guide RNA?

I want to know tutorial help to design and how to choose suitable g for knok out

29 July 2022 4,099 2 View

Which region of the gene should I target for a CRISPR mediated Knockout of Target gene?

I want to design g RNA for knock out pathway gene to enhance secondary metabolites

29 July 2022 8,077 0 View

What is the right media for HCT116?

Is DMEM or DMEM/F-12K the right media for culturing HCT116 cells apart from McCoys media? Did anyone use the gibco one? I see a lot of dead cells when using DMEM. It would be helpful if someone...

20 June 2022 1,059 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Where can I find free research instruments for Nursing?

for eg. transition shock scale

01 August 2024 5,998 0 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View