Hi,
I have labeled my sperm cell samples with FITC conjugated lectins and have captured files in Tiff formats. Now I want to measure fluorescent intensity by using IMAGE J software. According to the intensity measurement procedure, I'm following the same steps as mentioned in the IMAGE J manual. But I have one confusion before going to intensity measurement that what are the bases of the selection of 8 bit, 16 bit, 32 bit and RGB type to process tiff files for conversion into gray-scale? please suggest to me how and why I have to select the image type mentioned above???
Based on your query, for understanding 8, 16, 32,.... please follow: https://imagej.nih.gov/ij/docs/menus/image.html
Depends on the number of gray shades or color to be displayed. 8-bit images are used to display 256 gray shades or 256 colors (GIF files). RGB images utilize 2^24 or 16.7 million colors (standard photographic JPG files). 32-bit images add an extra 8-bit alpha channel (transparency information) to the normal 24-bit color images.
Depends on the number of gray shades or color to be displayed. 8-bit images are used to display 256 gray shades or 256 colors (GIF files). RGB images utilize 2^24 or 16.7 million colors (standard photographic JPG files). 32-bit images add an extra 8-bit alpha channel (transparency information) to the normal 24-bit color images.
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