Subhash Solanki

4 Questions 6 Answers 0 Followers

Questions related from Subhash Solanki

How access of dNTPs mixture inhibits Taq polymerase activity during a PCR reaction??

What is the relation between excess dNTPs and MgCl2? please comment.

05 January 2022 7,543 2 View

How to add bio-informatically the glycan/phosphate moieties at amino-acid in protein 3D (tertiary) structure?

Hi, I have found two SNPs (Alanine to serine & threonine) at different positions in my desired gene (Sanger sequencing), and then I have built a protein 3D (tertiary) structure by using...

07 July 2020 6,799 8 View

What are bases of selection of 8 bit, 16 bit, 32 bit and RGB image type during the intensity measurement (IMAGE J) of FITC-labeled cells?

Hi, I have labeled my sperm cell samples with FITC conjugated lectins and have captured files in Tiff formats. Now I want to measure fluorescent intensity by using IMAGE J software. According to...

06 February 2020 7,808 3 View

How to justify small size sample (N=5-7 only) in genomics?

Hi, I have started working with the two groups having characterized the small size of animal samples (N=5-7 animals each group) due to the limitation of the availability of samples. I got some...

04 January 2020 2,332 6 View