Hello everybody
In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+.
As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether you have alternatives for this supplement.
We already thought about normal B27, or the one from R&D (https://www.rndsystems.com/products/n21-max-media-supplement-50x-_ar008).
But in both of them the "+" seems to be missing.
Do you have any other ideas, or do you know which ingredients are needed to make B27+ out of B27?
Best wishes
Katharina
After running out of B27 from Thermo, we started using NCS21 from capricorn (essentially the same content) for mES cell differentiation to neurons and it worked the same as B27. You may check out the complete formulation here: https://www.capricorn-scientific.com/capricorn/documents/product%20information/Cell%20Culture%20Reagents/Product%20Info_C21-H.pdf for more details. If you mean retinyl acetate by "+" this sure has it. Manufacturer's usually signify "minus vitamin A" if it is not "+".
Best,
Onur
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