1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to get nanocomposite of PEEK with Zirconia ?

Need a lengthy PEEK with Zirconia based nano composite of 180 mm length. Which method is possible? Tried injection molding no proper mix. Solvent casting process is also very costly. Kindly anyone...

13 December 2020 4,329 5 View

How can I get eggs of an univoltine casebearer moth for a threshold study?

I am planning to conduct a cage study to determine a critical density of an univoltine casebearer moth in red clover seed crop. This requires to release first instar larvae in the cages with the...

02 November 2020 6,577 3 View

Can anyone suggest me free and fast scopus publication with highly acceptance rate?

Q3,Q4 will be okay. I need scopus publication without APC and fast published. The paper is from Computer science discipline.

19 October 2020 4,255 4 View

Bio-intensive cropping systems can reduce the environmental foot print?

The adoption of bio-intensive cropping systems has the potential to reduce environmental footprints without compromising the economic gain per unit of resources.

12 October 2020 2,625 7 View

Application of Polyhalite changes the nutrients chemistry in soil?

Polyhalite is a multi-nutrient source, its application assume to be changes the soil biology and nutrients chemistry on long term basis

12 October 2020 6,685 3 View

How to recycle Sodium Iodide?

Hi, I am using sodium iodide to separate density carbon fractions of my samples. I want to recyle it for further uses. While searching I only get the process to recycle sodium polytungstate (at...

20 September 2020 9,556 2 View

Purpose of keeping the rocket nozzle assembly inverted, for Actuator-In-Loop simulations ?

For Actuator-In-Loop simulations, why are the rocket engine nozzles along with the thrust vector control assembly (including the actuators) kept inverted on a platform like in the attached image...

14 August 2020 8,652 2 View

How to add attenuation model and spectral ordinate in R-CRISIS?

Hi Everyone I am using R-CRISIS for seismic hazard map creation. I know how to use the given attenuation model in R-CRISIS but I want to use an attenuation model from a published article. How to...

23 July 2020 5,265 1 View

Is there any python module is available for statistical seismology?

Could you suggest any python module that is made for dealing with statistical seismology?

29 May 2020 9,068 6 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View