I am trying to see if I can test the serum of infected mice with this antibody
Hello Carolina: antibody IGG posative with diagnosis of E. Histolytica.
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Thank you! Intisar R. Sharba and Qt Ping
Can lymphocytes be used to grow toxoplasmosis?
The IgA is responsible for the initial immune response to infection
Hi, I agree with Sukayna Jabbar answer
What is the best way to eliminate cutaneous leishmaniasis
I have tried bead-beating and freeze thawing methods with no luck, yet Thanks!
05 June 2019 10,091 2 View
Hi guys, I am trying to determine how my parasites I have in a sample using a standard curve of trophs. I am looking at the 18S gene, but for the normalization I am thinking copy number is...
02 March 2019 3,018 1 View
Will be attempting to pass some parasites in vivo (suseptible mice) and was wondering what is considered acceptable in the field.
07 August 2018 3,994 5 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
I am working on a project proposal (DFG project in Germany). I have the problem that I want to calculate how much funds I have to request for my test subjects (e.g., for qualitative interview of...
18 July 2024 1,690 0 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View