Hi guys,
I am trying to determine how my parasites I have in a sample using a standard curve of trophs. I am looking at the 18S gene, but for the normalization I am thinking copy number is important.
Thanks in advance,
Caro
Have you tried qPCR? If not, is a way to go.
Hope it helps.
I have tried bead-beating and freeze thawing methods with no luck, yet Thanks!
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