Checking cytotoxic effects on cancer cell lines is a usual preliminary test for anti-cancer activity. The MTT assay can be used for that.

You can also have a look on the following discussion on anti-proliferative tests:

https://www.researchgate.net/post/Is_an_MTT_assay_sufficient_to_check_antiproliferative_activity_of_some_clinically_useful_drugs/1

Thanks Yahaira

I had done the MTT and what to do further is the thing I am looking for .

I think The papers you have suggested may help

Let me look into it.

Will keep in touch if I have some doubt after that

Thank you

Thanks Christine :)

Interesting anti-cancer effects also relate to other properties, such as anti-migratory and anti-angiogenic effects.

The literature describes high-throughput assays for testing these aspects for which 3D assays are particularly recommended.