I want to culture human HepG2 cells in serum free medium. Should I add all components of complete medium except FBS only?, and how long the cells will be alive in such free medium?
Greetings,
1) For serum-free culture, I used basal medium only without antibiotics and serum. An article you could refer to : Article Stem Cell Secretome and Its Effect on Cellular Mechanisms Re...
2) For the incubation time, it depends on the cell type however, most studies have reported that cells remain viable up to 16h-24h. Since HepG2 is an immortalized cell line, there would most probably be no viability problem. You could perform cell viability analysis in the basal medium at different time points, say 8h, 16h and 24h. A simple MTT assay would do the trick. Good luck.
If you need an alternative to FBS, you can always consider human platelet lysate. Here's a link if you are interested in trying it out: https://www.bioind.com/pltgold-human-platelet-lysate/
Generally if you are avoiding FBS and you want to keep your culture serum-free, HPL works really well.
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