How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can the intensity of this broad band in XRD mask other crystaline phases present?

I obtained a XRD diffractogram with significant broad band at low 2theta levels, also around 30 2theta another smaller broad band appears. It all points out to the existence of an amorphous phase....

19 February 2021 8,414 4 View

Sol-gel synthesis of 45S5?

I've synthesized 45S5 bioglass by sol-gel method and my XRD and FTIR results are similar to 45S5 bioglass but my EDX result has differences and instead of Si and Na the amount of Ca is higher....

29 December 2020 4,847 2 View

Why the theoretical calculation of percentage atom varies from measured data from EDX study?

I am wondering why there is a variation in the theoretical atomic percentage in Ni-Cu ferrites and the percentage atom values found from EDX spectra. In the case of Fe and O, there exists this...

25 November 2020 2,470 3 View

How I can relate EDX and XRD data of a ceramic based compound?

I have taken EDX of a ceramic compound and It has shown all the expected elements along with different weight and atom percentage. But in XRD data of the same compound, all the elements have not...

23 November 2020 2,144 10 View

Is there any technique to reduce carbonate precentage in bone sample (bovine hydroxyapatite)?

Dear colleagues, I am a researcher working with bovine hydroxyapatite. I want to reduce the percentage of carbonate on the sample. Is there any suggested techniques for this? I am measured the...

19 November 2020 3,828 3 View

How can we confirm the formation of solid solution by using EDX point analysis and elemental mapping ?

I fabricated a composite say AB and both are carbides ceramics having formula AC and BC. I did EDX point analysis and observed A (45 wt %) and B (28 wt %), Carbon (26 wt %) and Oxygen (1 wt %)....

20 October 2020 7,419 7 View

How can I false color of SEM images (tiff format) ?

I have SEM image of cross section of multilayer thin film sample and need to represent the different color for different layer without losing its morphological information on the image. Is there...

08 October 2020 9,764 6 View

Can protons, ions and neutrons be used for SEM imaging rather than electron beam?

Generally it is known that SEM is based on the principle of interaction of electron beam with matter. Proton and ion beam can be used instead of electron beam? How their interaction will be...

27 September 2020 4,432 9 View

Why I observe the Si peak after analyzing PEO on EDX?

After putting the PEO coated glass substrate into the oven and subjecting it to a temperature of 180 degrees, it obtains a haze on the glass. A peak Si was observed after EDX analysis. Is there...

24 September 2020 6,335 6 View

Why material with same structure and composition performs differently in RAMAN Spectroscopy?

I was carrying out RAMAN Spectroscopy for MOFs. I have been carried out the XRD and EDX analysis for three samples synthesized by a different protocol. The crystal structure and chemical...

13 September 2020 7,167 5 View