How can I corroborate the integrity por exosome-derived small RNA?

Hi everyone! I work with Mesenchymal Stem Cells-derived exosomes and I am interested in doing next generation sequencing of exosome-derived small RNAs. After isolation, I obtain a very small...

13 January 2020 7,123 3 View

Transwell Tregs migration assay: fibronectin coating?

I performed this assay many times but without any consistent result. I usually isolate mouse spleen CD4+CD25+ cells, seed 100K non-activated cells on 3um-pore size membranes (24well plate,...

08 November 2019 3,030 1 View

Method for statistical analysis of semiquantitative Western-Blot?

Hello, I need some advice about how to statistically analyze WB data. I am trying to compare the levels of two proteins in mutants relative to wt samples (it's mouse embryonic tissue). I...

10 February 2019 6,052 3 View

How long does it take to an article being cited?

It's depend of knowledge areas or researcher's visibility.

12 February 2018 7,065 7 View

How long does it take to an article being cited?

It's depend of knowledge areas or researcher's visibility.

01 February 2018 4,230 6 View

Where can I buy thermolastic strach (TPS)?

I am looking for a distributer that currently have TPS in stock, and where I could buy a small quantity for research purposes with which I could used to make composites..

12 December 2017 3,118 3 View

In your opinion, does science respond to specific contexts or does science respond to scientometric indicators?

Question about new conditions which researchers are facing, i will appreciate your contribution.

11 December 2017 3,438 3 View

PLA/cellulose fiber composites, what are the optimal mass concentration for improved mechanical properties?

01 October 2017 336 2 View

What is the maximum amount of SNPs that a strain had to have to be consider isogenic with other?

21 June 2017 2,174 3 View

How liver RNA extract?

Immediately frozen liver with dry ice, I cut a piece of 100mg, I add 1 ml of Trizol reagent, homogenized with homogenizer power and proceed to phase separation, precipitation and RNA wash...

02 November 2016 7,462 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,206 4 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View