Which fluorescent filter is required for observing GFP encoded protein in bacterial cells ?

GFP is encoded in bacterial cell, which filter is required to observe such cells under a fluorescence microscope?

21 January 2020 2,561 3 View

Can we analyse the morphology of liposomes by Scanning Electron Microscopy (SEM)?

Can we analyse the morphology of liposomes by Scanning Electron Microscopy (SEM)?

19 July 2019 9,874 4 View

How to do in vitro enzymatic hydrolysis study of lipophilic prodrugs?

We have to carry out in vitro hydrolysis of lipophilic prodrug (log P 3.39) to drug in the presence of enzymes. As the drug is having very poor solubility in aqueous phase (phosphate buffer pH...

26 September 2018 6,971 2 View

How to clone the 3' UTR of gene whose length is very small?

3' UTR of my gene of interests spans in a total of around 120 bp, although the 5' UTR is huge in kbs. I am not able to design suitable primers to clone the whole UTR for luc-assay. Tried making...

23 July 2018 6,889 4 View

How to "equalize" these two data plots?

I have two data from two different sensors (Blue = Sensor 1 and Red = Sensor 2) 1.png . Sensor 2 is the gold standard and I wish to recalibrate sensor 1 so as to get an "equalised" data plot like...

19 June 2016 2,750 3 View

What are the sources /causes of channel estimation error (CEE) ?

06 April 2016 1,772 2 View

My cell line U87MG is getting detached after splitting, what could be the reason?

Few weeks back there was fungal infection in some of my petri dishes. So, I thoroughly cleaned the incubator using bleach and rectified alcohol, revived fresh stocks, prepared everything fresh and...

24 August 2015 9,535 16 View

How can I hybridize the Error Back Propogation algorithm with the Genetic algorithm in Matlab?

I am using this method to predict the stock market.

16 March 2015 8,098 2 View

Can we perform flow cytometry on -80 frozen samples?

We collected the plants 2 months back and stored them in -80

26 April 2014 8,841 4 View

Under which filter should I view acridine orange and Etbr staining for checking cell viability of cancer cells?

I just want to know the cells should be viewed under which filter.

15 April 2014 1,549 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View